Aldehyde dehydrogenase 2 and glutathione S-transferase M 1 polymorphisms in relation to the risk for oral cancer in Japanese drinkers

Nomura, T; Noma, H; Shibahara, T; Yokoyama, A; Muramatusu, T; Ohmori, T

« Previous Next »Oral Oncology
Volume 36, Issue 1 , Pages 42-46, January 2000

Aldehyde dehydrogenase 2 and glutathione S-transferase M 1 polymorphisms in relation to the risk for oral cancer in Japanese drinkers

T Nomura
- The First Department of Oral and Maxillofacial Surgery, Tokyo Dental College, 1-2-2 Masago, Mihama-ku, Chiba 261-0011, Japan
H Noma
- The First Department of Oral and Maxillofacial Surgery, Tokyo Dental College, 1-2-2 Masago, Mihama-ku, Chiba 261-0011, Japan
T Shibahara
- The First Department of Oral and Maxillofacial Surgery, Tokyo Dental College, 1-2-2 Masago, Mihama-ku, Chiba 261-0011, Japan
- Corresponding author. Tel.: +81-43-270-3973
A Yokoyama
- National Institute on Alcoholism, Kurihama National Hospital, 5-3-1 Nobi, Yokosuka-shi, Kanagawa, 239-0841, Japan
T Muramatusu
- Department of Nueropsychiatry, Keio University, 35 Shinano-cho, Shinjyuku-ku, Tokyo, 160-0016, Japan
T Ohmori
- Department of Surgery, Municipal Kawasaki Hospital, 12-1 Shinkawadori, Kawasaki-ku, Kawasaki-shi, Kanagawa, 210-0013, Japan

Received 23 March 1999; received in revised form 18 May 1999; accepted 18 May 1999.

Abstract

We investigated 191 patients with oral cancer (121 males and 70 females) and 121 non oral cancer patients (69 males and 52 females), both groups with a history of alcohol use. Blood was analyzed with aldehyde dehydrogenase 2 (ALDH 2) and glutathione S-transferase M 1 (GSTM 1) genotyping. ALDH 2 genotyping was performed by polymerase chain reaction (PCR)–Restriction fragment length polymorphism (RFLP) method and GSTM 1 genotyping was amplified with PCR using GSTM 1 specific primers. In the oral cancer group, the alcohol-drinking rate (59.7%) was significantly higher than in the non cancer group (alcohol-drinking rate 27.3%, p<0.01). The incidence of inactive ALDH 2 and GSTM 1 in the cancer group with an alcohol-drinking habit was 34.2 and 67.5% and was higher than in the non cancer group with an alcohol-drinking habit (15.1, 45.5%).

Keywords: Genotypic polymorphisms, Oral cancer, Aldehyde dehydrogenase 2, Glutathione S-transferase M 1, Drinking habit