Oral squamous cell carcinoma detection by salivary biomarkers in a Serbian population

Brinkmann, Ole; Kastratovic, Dragana A.; Dimitrijevic, Milovan V.; Konstantinovic, Vitomir S.; Jelovac, Drago B.; Antic, Jadranka; Nesic, Vladimir S.; Markovic, Srdjan Z.; Martinovic, Zeljko R.; Akin, David; Spielmann, Nadine; Zhou, Hui; Wong, David T.

doi:10.1016/j.oraloncology.2010.10.009

« Previous Next »Oral Oncology
Volume 47, Issue 1 , Pages 51-55, January 2011

Oral squamous cell carcinoma detection by salivary biomarkers in a Serbian population

Ole Brinkmann
- School of Dentistry and Dental Research Institute, University of California – Los Angeles, 10833 Le Conte Avenue, 73-029 CHS, Los Angeles, CA 90095, USA
- Joint first authors.
Dragana A. Kastratovic
- Clinical Center of Serbia, Pasterova 2, 1100 Belgrade, Serbia
- Joint first authors.
Milovan V. Dimitrijevic
- Clinical Center of Serbia, Pasterova 2, 1100 Belgrade, Serbia
Vitomir S. Konstantinovic
- Stomatology Faculty University of Belgrade, Dr. Subotica 4, 11000 Belgrade, Serbia
Drago B. Jelovac
- Stomatology Faculty University of Belgrade, Dr. Subotica 4, 11000 Belgrade, Serbia
Jadranka Antic
- Clinical Center of Serbia, Pasterova 2, 1100 Belgrade, Serbia
Vladimir S. Nesic
- Clinical Center of Serbia, Pasterova 2, 1100 Belgrade, Serbia
Srdjan Z. Markovic
- Medical Faculty University of Belgrade, Dr. Subotica 4, 11000 Belgrade, Serbia
Zeljko R. Martinovic
- Stomatology Faculty University of Belgrade, Dr. Subotica 4, 11000 Belgrade, Serbia
David Akin
- School of Dentistry and Dental Research Institute, University of California – Los Angeles, 10833 Le Conte Avenue, 73-029 CHS, Los Angeles, CA 90095, USA
Nadine Spielmann
- School of Dentistry and Dental Research Institute, University of California – Los Angeles, 10833 Le Conte Avenue, 73-029 CHS, Los Angeles, CA 90095, USA
Hui Zhou
- School of Dentistry and Dental Research Institute, University of California – Los Angeles, 10833 Le Conte Avenue, 73-029 CHS, Los Angeles, CA 90095, USA
David T. Wong
- School of Dentistry and Dental Research Institute, University of California – Los Angeles, 10833 Le Conte Avenue, 73-029 CHS, Los Angeles, CA 90095, USA
- Jonsson Comprehensive Cancer Center, University of California – Los Angeles, 10833 Le Conte Avenue, Los Angeles, CA 90095, USA
- Division of Head and Neck Surgery/Otolaryngology, David Geffen School of Medicine, University of California – Los Angeles, 10833 Le Conte Avenue, Los Angeles, CA 90095, USA
- Henry Samueli School of Engineering and Applied Science, University of California – Los Angeles, 7400 Boelter Hall, Los Angeles, CA 90095, USA
- Corresponding author at: University of California at Los Angeles, School of Dentistry, Dental Research Institute, 73-017 CHS, 10833 Le Conte Avenue, Los Angeles, CA 90095, USA. Tel.: +1 310 206 3048; fax: +1 310 825 7609.

Received 28 September 2010; received in revised form 18 October 2010; accepted 19 October 2010. published online 25 November 2010.

Summary

Early detection of oral squamous cell cancer (OSCC) is the key to improve the low 5-year survival rate. Using proteomic and genomic technologies we have previously discovered and validated salivary OSCC markers in American patients. The question arises whether these biomarkers are discriminatory in cohorts of different ethnic background. Six transcriptome (DUSP1, IL8, IL1B, OAZ1, SAT1, and S100P) and three proteome (IL1B, IL8, and M2BP) biomarkers were tested on 18 early and 17 late stage OSCC patients and 51 healthy controls with quantitative PCR and ELISA. Four transcriptome (IL8, IL1B, SAT1, and S100P) and all proteome biomarkers were significantly elevated (p<0.05) in OSCC patients. The combination of markers yielded an AUC of 0.86, 0.85 and 0.88 for OSCC total, T1–T2, and T3–T4, respectively. The sensitivity/specificity for OSCC total was 0.89/0.78, for T1–T2 0.67/0.96, and for T3–T4 0.82/0.84. In conclusion, seven of the nine salivary biomarkers (three proteins and four mRNAs) were validated and performed strongest in late stage cancer. Patient-based salivary diagnostics is a highly promising approach for OSCC detection. This study shows that previously discovered and validated salivary OSCC biomarkers are discriminatory and reproducible in a different ethnic cohort. These findings support the feasibility to implement multi-center, multi-ethnicity clinical trials towards the pivotal validation of salivary biomarkers for OSCC detection.