Association of T-cell regulatory gene polymorphisms with oral squamous cell carcinoma

Kämmerer, Peer W.; Toyoshima, Takeshi; Schöder, Fabian; Kämmerer, Philipp; Kuhr, Kathrin; Brieger, Jürgen; Al-Nawas, Bilal

doi:10.1016/j.oraloncology.2010.03.025

« Previous Next »Oral Oncology
Volume 46, Issue 7 , Pages 543-548, July 2010

Association of T-cell regulatory gene polymorphisms with oral squamous cell carcinoma

Peer W. Kämmerer
- Department of Oral and Maxillofacial Surgery, University Medical Centre of the Johannes Gutenberg University, Mainz, Germany
- Authors contributed equally.
Takeshi Toyoshima
- Department of Oral and Maxillofacial Surgery, University Medical Centre of the Johannes Gutenberg University, Mainz, Germany
- Department of Oral and Maxillofacial Surgery, Kyushu University, Japan
- Corresponding author at: Department of Oral and Maxillofacial Surgery, Johannes-Gutenberg University, Augustusplatz 2, 55131 Mainz, Germany. Tel.: +49 (0) 6131 17 5086; fax: +49 (0) 6131 17 6602.
- Authors contributed equally.
Fabian Schöder
- Department of Oral and Maxillofacial Surgery, University Medical Centre of the Johannes Gutenberg University, Mainz, Germany
Philipp Kämmerer
- Department of Oral and Maxillofacial Surgery, University Medical Centre of the Johannes Gutenberg University, Mainz, Germany
Kathrin Kuhr
- Institute of Medical Biostatistics, Epidemiology and Informatics (IMBEI), University Medical Centre of the Johannes Gutenberg University, Mainz, Germany
Jürgen Brieger
- Department of Otorhinolaryngology, Head and Neck Surgery, Molecular Tumor Biology Laboratory, University Medical Centre of the Johannes Gutenberg University, Mainz, Germany
Bilal Al-Nawas
- Department of Oral and Maxillofacial Surgery, University Medical Centre of the Johannes Gutenberg University, Mainz, Germany

Received 4 February 2010; received in revised form 30 March 2010; accepted 30 March 2010. published online 03 May 2010.

Summary

Costimulatory molecules have complementary effects on T-cell activation and their balance may control the development of oral cancer. The aim of this study was to determine the relevance of cytotoxic T-lymphocyte antigen 4 (CTLA-4), CD28 and inducible costimulator (ICOS) polymorphisms in oral squamous cell carcinoma (OSCC).

Genotyping for CTLA-4 (−1661 A/G and +49 A/G), CD28 (0 C/G and +3160 G/T) and ICOS (+637 A/C and +1599 C/T) was performed in the 83 patients with OSCC, compared to the 40 unrelated healthy volunteers as controls.

The genotype CTLA-4 −1661 was significantly different between the patient group and the control group. The allele CTLA-4 −1661 G was significantly found more frequent in patients with OSCC (p=0.001). In bivariate analysis, noticeable differences between OSCC and controls were seen. The combinations CTLA-4 −1661 G/G and CTLA-4 +49 A/G, ICOS +1559 C/T and ICOS +1559 C/C each with CTLA-4 −1661 G/G, ICOS +637 C/C and ICOS +637 A/C each with CTLA-4 −1661, CTLA-4 −1661 A/G and ICOS +637 C/C, CD28 +3160 G/T and CTLA-4 −1661 A/A and CD28 +3160 G/T and CTLA-4 −1661 A/G were seen in the patient group only.

Especially the polymorphisms of the CTLA-4 −1661-genotype – alone and in combination with other T cell regulator polymorphisms – seem to be possible predisposing factors for OSCC. Therefore, they might be future targets for a primary prophylaxis or an individualised therapy.

Keywords: Oral squamous cell carcinoma, T-lymphocyte, Polymorphism, Costimulatory molecule, CTLA-4, CD28, ICOS, Case–control