miR-24 up-regulation in oral carcinoma: Positive association from clinical and in vitro analysis

Lin, Shu-Chun; Liu, Chung-Ji; Lin, Jung-An; Chiang, Wei-Fan; Hung, Pei-Shih; Chang, Kuo-Wei

doi:10.1016/j.oraloncology.2009.12.005

« Previous Next »Oral Oncology
Volume 46, Issue 3 , Pages 204-208, March 2010

miR-24 up-regulation in oral carcinoma: Positive association from clinical and in vitro analysis

Shu-Chun Lin
- School of Dentistry, National Yang-Ming University, Taipei, Taiwan
Chung-Ji Liu
- School of Dentistry, National Yang-Ming University, Taipei, Taiwan
- Oral and Maxillofacial Surgery, MacKay Memorial Hospital, Taipei, Taiwan
Jung-An Lin
- School of Dentistry, National Yang-Ming University, Taipei, Taiwan
Wei-Fan Chiang
- School of Dentistry, National Yang-Ming University, Taipei, Taiwan
- Department of Dentistry, Chimei Hospital, Liouying Campus, Tainan, Taiwan
Pei-Shih Hung
- School of Dentistry, National Yang-Ming University, Taipei, Taiwan
Kuo-Wei Chang
- School of Dentistry, National Yang-Ming University, Taipei, Taiwan
- Department of Stomatology, Veterans General Hospital, Taipei, Taiwan
- Corresponding author. Address: School of Dentistry, National Yang-Ming University, Beitou, Taipei 112, Taiwan. Tel.: +886 2 28267223; fax: +886 2 28264053.

Received 1 December 2009; received in revised form 16 December 2009; accepted 17 December 2009. published online 08 February 2010.

Summary

MicroRNAs (miRNAs) play important roles in neoplastic process. miR-24 is localized on chromosome 9q22 and 19p13, regions frequently altered in oral squamous cell carcinoma (OSCC). This study showed that miR-24 was up-regulated in OSCC tissues relative to control samples. In addition, the plasma levels of miR-24 in OSCC patients were significantly higher than in control individuals. miR-24 expression was also higher in OSCC cell lines relative to normal oral keratinocytes. Experiments blocking miR-24 and using exogenous miR-24 expression indicated that miR-24 contributes to the growth of OSCC cells and that miR-24 may target p57. This study suggests that miR-24 is involved in the regulation of OSCC growth and that the miR-24’s level in plasma may be validatable as a tumor marker for OSCC patients.

Keywords: Carcinoma, MicroRNA, Oral cavity, Plasma, Proliferation, miR-24