Oral Oncology
Volume 46, Issue 3 , Pages 166-171 , March 2010

Downregulation of CD9 protein expression is associated with aggressive behavior of oral squamous cell carcinoma

  • Marcilei Eliza Cavicchioli Buim

      Affiliations

    • Department of Anatomical Pathology, Hospital A.C. Camargo, São Paulo, Brazil
    • Corresponding Author InformationCorresponding author. Address: Department of Anatomical Pathology, Hospital A.C. Camargo, 109 Professor Antônio Prudente St., Zip Code 01509-900, São Paulo, Brazil. Tel./fax: +55 11 21895185.
  • ,
  • Silvia Vanessa Lourenço

      Affiliations

    • Department of General Pathology, Dental School, University of São Paulo, Brazil
  • ,
  • Kátia Candido Carvalho

      Affiliations

    • Department of Anatomical Pathology, Hospital A.C. Camargo, São Paulo, Brazil
  • ,
  • Roberta Cardim

      Affiliations

    • Department of Head and Neck Surgery, Hospital A.C. Camargo, São Paulo, Brazil
  • ,
  • Cláudia Pereira

      Affiliations

    • Department of Head and Neck Surgery, Hospital A.C. Camargo, São Paulo, Brazil
  • ,
  • André Lopes Carvalho

      Affiliations

    • Department of Head and Neck Surgery, Barretos Cancer Hospital, São Paulo, Brazil
  • ,
  • José Humberto Fregnani

      Affiliations

    • Department of Gynecological Oncology, Barretos Cancer Hospital, São Paulo, Brazil
  • ,
  • Fernando Augusto Soares

      Affiliations

    • Department of Anatomical Pathology, Hospital A.C. Camargo, São Paulo, Brazil
    • Department of General Pathology, Dental School, University of São Paulo, Brazil

Received 25 September 2009 ,Revised 19 November 2009 ,Accepted 20 November 2009.

References 

  1. Kawano K, Yanagisawa S. Predictive value of laminin-5 and membrane type 1-matrix metalloproteinase expression for cervical lymph node metastasis in T1 and T2 squamous cell carcinomas of the tongue and floor of the mouth. Head Neck. 2006;28(6):525–533
  2. Longo N, Yanez-Mo M, Mittelbrunn M, de la Rosa G, Munoz ML, Sanchez-Madrid F, et al. Regulatory role of tetraspanin CD9 in tumor–endothelial cell interaction during transendothelial invasion of melanoma cells. Blood. 2001;98(13):3717–3726
  3. Erovic BM, Pammer J, Hollemann D, Woegerbauer M, Geleff S, Fischer MB, et al. Motility-related protein-1/CD9 expression in head and neck squamous cell carcinoma. Head Neck. 2003;25(10):848–857
  4. van Spriel AB, Puls KL, Sofi M, Pouniotis D, Hochrein H, Orinska Z, et al. A regulatory role for CD37 in T cell proliferation. J Immunol. 2004;172(5):2953–2961
  5. Levy S, Todd SC, Maecker HT. CD81 (TAPA-1): a molecule involved in signal transduction and cell adhesion in the immune system. Annu Rev Immunol. 1998;16:89–109
  6. Saito Y, Tachibana I, Takeda Y, Yamane H, He P, Suzuki M, et al. Absence of CD9 enhances adhesion-dependent morphologic differentiation, survival, and matrix metalloproteinase-2 production in small cell lung cancer cells. Cancer Res. 2006;66:9557–9565
  7. Huang CI, Kohno N, Ogawa E, Adachi M, Taki T, Miyake M. Correlation of reduction in MRP-1/CD9 and KAI1/CD82 expression with recurrences in breast cancer patients. Am J Pathol. 1998;153(3):973–983
  8. Boucheix C, Rubinstein E. Tetraspanins. Cell Mol Life Sci. 2001;58(9):1189–1205
  9. Mhawech P, Herrmann F, Coassin M, Guillou L, Iselin CE. Motility-related protein 1 (MRP-1/CD9) expression in urothelial bladder carcinoma and its relation to tumor recurrence and progression. Cancer. 2003;98(8):1649–1657
  10. Sauer G, Windisch J, Kurzeder C, Heilmann V, Kreienberg R, Deissler H. Progression of cervical carcinomas is associated with down-regulation of CD9 but strong local re-expression at sites of transendothelial invasion. Clin Cancer Res. 2003;9(17):6426–6431
  11. Kusukawa J, Ryu F, Kameyama T, Mekada E. Reduced expression of CD9 in oral squamous cell carcinoma: CD9 expression inversely related to high prevalence of lymph node metastasis. J Oral Pathol Med. 2001;30(2):73–79
  12. Pfaffl MW. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 2001;29:45
  13. Adachi M, Taki T, Konishi T, Huang CI, Higashiyama M, Miyake M. Novel staging protocol for non-small-cell lung cancers according to MRP-1/CD9 and KAI1/CD82 gene expression. J Clin Oncol. 1998;16(4):1397–1406
  14. Sho M, Adachi M, Taki T, Hashida H, Konishi T, Huang CL, et al. Transmembrane 4 superfamily as a prognostic factor in pancreatic cancer. Int J Cancer. 1998;79(5):509–516
  15. Chen Z, Mustafa T, Trojanowicz B, Brauckhoff M, Gimm O, Schmutzler C, et al. CD82 and CD63 in thyroid cancer. Int J Mol Med. 2004;14(4):517–527
  16. Hotta H, Ross AH, Huebner K, Isobe M, Wendeborn S, Chao MV, et al. Molecular cloning and characterization of an antigen associated with early stages of melanoma tumor progression. Cancer Res. 1988;48(11):2955–2962
  17. Radford KJ, Mallesch J, Hersey P. Suppression of human melanoma cell growth and metastasis by the melanoma-associated antigen CD63 (ME491). Int J Cancer. 1995;62(5):631–635
  18. Ikeyama S, Koyama M, Yamaoko M, Sasada R, Miyake M. Suppression of cell motility and metastasis by transfection with human motility-related protein (MRP-1/CD9) DNA. J Exp Med. 1993;177(5):1231–1237
  19. Dong JT, Lamb PW, Rinker-Schaeffer CW, Vukanovic J, Ichikawa T, Isaacs JT, et al. KAI1, a metastasis suppressor gene for prostate cancer on human chromosome 11p11.2. Science. 1995;268(5212):884–886
  20. Yu Y, Yang JL, Markovic B, Jackson P, Yardley G, Barrett J, et al. Loss of KAI1 messenger RNA expression in both high-grade and invasive human bladder cancers. Clin Cancer Res. 1997;3(7):1045–1049
  21. Lombardi DP, Geradts J, Foley JF, Chiao C, Lamb PW, Barrett JC. Loss of KAI1 expression in the progression of colorectal cancer. Cancer Res. 1999;59(22):5724–5731
  22. Uchida S, Shimada Y, Watanabe G, Li ZG, Hong T, Miyake M, et al. Motility-related protein (MRP-1/CD9) and KAI1/CD82 expression inversely correlate with lymph node metastasis in oesophageal squamous cell carcinoma. Br J Cancer. 1999;79(7–9):1168–1173
  23. Miyazaki T, Kato H, Shitara Y, Yoshikawa M, Tajima K, Masuda N, et al. Mutation and expression of the metastasis suppressor gene KAI1 in esophageal squamous cell carcinoma. Cancer. 2000;89(5):955–962
  24. Liu FS, Dong JT, Chen JT, Hsieh YT, Ho ES, Hung MJ, et al. KAI1 metastasis suppressor protein is down-regulated during the progression of human endometrial cancer. Clin Cancer Res. 2003;9(4):1393–1398
  25. Uzawa K, Ono K, Suzuki H, Tanaka C, Yakushiji T, Yamamoto N, et al. High prevalence of decreased expression of KAI1 metastasis suppressor in human oral carcinogenesis. Clin Cancer Res. 2002;8(3):828–835
  26. Farhadieh RD, Smee R, Ow K, Yang JL, Russell PJ, Crouch R, et al. Down-regulation of KAI1/CD82 protein expression in oral cancer correlates with reduced disease free survival and overall patient survival. Cancer Lett. 2004;213(1):91–98
  27. Imai Y, Sasaki T, Shinagawa Y, Akimoto K, Fujibayashi T. Expression of metastasis suppressor gene (KAI1/CD82) in oral squamous cell carcinoma and its clinico-pathological significance. Oral Oncol. 2002;38(6):557–561
  28. Sakamoto K, Nakamura Y, Nakashima T. Immunohistochemical distribution of CD9 in parotid gland tumors. Auris Nasus Larynx. 2004;31(1):49–55
  29. Miyamoto S, Maruyama A, Okugawa K, Akazawa K, Baba H, Maehara Y, et al. Loss of motility-related protein 1 (MRP1/CD9) and integrin alpha3 expression in endometrial cancers. Cancer. 2001;92(3):542–548
  30. Funakoshi T, Tachibana I, Hoshida Y, et al. Expression of tetraspanins in human lung cancer cells: frequent downregulation of CD9 and its contribution to cell motility in small cell lung cancer. Oncogene. 2003;22(5):674–687
  31. Wang JC, Begin LR, Berube NG, Chevalier S, Aprikian AG, Gourdeau H, et al. Down-regulation of CD9 expression during prostate carcinoma progression is associated with CD9 mRNA modifications. Clin Cancer Res. 2007;13(18):2354–2361

 Supported by the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), Brazil.

PII: S1368-8375(09)00978-6

doi: 10.1016/j.oraloncology.2009.11.009

Oral Oncology
Volume 46, Issue 3 , Pages 166-171 , March 2010