Expression of annexin A1 mRNA in peripheral blood from oral squamous cell carcinoma patients
Received 29 July 2009; received in revised form 7 September 2009; accepted 7 September 2009. published online 15 October 2009.
Summary
Several studies have been suggesting annexin A1 protein as an active player in tumorigenesis of many organs. Nevertheless, its tumor biomarker role has been mainly studied in tissues by immunohistochemistry or cell culture. Hence, in this investigation, the peripheral blood from 27 oral squamous cell carcinoma (OSCC) patients and 25 negative control individuals were examined by quantitative real-time PCR. Down-regulated ANXA1 expression at mRNA level was observed in OSCC samples (p=0.026). Significantly diminished mRNA levels correlated to age, sex and the anatomical site of the tumor lesion were observed. Moreover, the ROC curve analysis revealed the performance of ANXA1 expression as a suitable biomarker for patients with oral cavity cancer, especially those with 60years of age or older and/or women. For the first time, ANXA1 mRNA is revealed as blood-based biomarker, and its adoption for complementary non-invasive diagnosis of OSCC is suggested. These results suggest that, beyond the anti-inflammatory function, annexin A1 may also play a tumor suppressor role in peripheral blood cells, such as leukocytes.
aNanobiotechnology Laboratory, Institute of Genetics and Biochemistry, Federal University of Uberlândia, Av. Amazonas, Bloco 2E, Campus Umuarama, CEP 38400-902 Uberlândia, MG, Brazil
bOral Pathology Laboratory, Clinics’ Hospital, Federal University of Uberlândia, MG, Brazil
cHead and Neck Service, Clinics’ Hospital, Federal University of Uberlândia, MG, Brazil
dStomatology Diagnosis Unit, Faculty of Dentistry, Federal University of Uberlândia, MG, Brazil
eImmunomorphology Laboratory, Department of Biology, Instituto de Biociências, Letras e Ciências Exatas (IBILCE), UNESP, Av. Cristóvão Colombo, 2265, CEP 15054-000 São José do Rio Preto, SP, Brazil
ABSTRACT