Erratum to “Poster session III: Basic science, epidemiology-diagnosis, radiation injuries” [Oral Oncology Supplement 3 (2009) 201–236]

Article Outline

 

It is with regret that the authors names have incorrectly appeared in some abstracts. The relevant pages have now been reproduced on the corrected following pages.

P3.05. Oral cancer susceptibility, metastasis, and recurrence in relation to Tenascin C genotypes

D.T. Bau^a,b,*, H.C. Tseng^a, C.H. Wang^a, C.F. Chiu^a, C.N. Wu^a,c, Ming-Hsui Tsai^a

^aTerry Fox Cancer Research Laboratory, China Medical University Hospital, Taichung, Taiwan

^bGraduate Institute of Chinese Medical Science, China Medical University, Taichung, Taiwan

^cInstitute of Medical Bioscience, Central-Taiwan University of Science and Technology, Taichung, Taiwan, ROC

Tenascin C (TNC) is a mosaic, linear glycoprotein that is up-regulated during many normal and pathological processes involving either cell migration or tissue morphogenesis, such as invasion of malignant cells and wound healing. It is expressed in invasive human solid tumors; however its specific role in cancer biology remains obscure. In this study, we firstly hypothesized that single nucleotide polymorphisms (SNPs) in TNC may be associated with risk of oral cancer. In this hospital-based study, the association of TNC A2128G (rs3827816), C2354T (rs1061494), T5344A (rs2104772), G6337C (rs13321) and intron15 (rs1537017) polymorphisms with oral cancer risk in a central Taiwanese population was investigated. In total, 696 patients with oral cancer and 696 age- and gender-matched healthy controls recruited from the China Medical Hospital in central Taiwan were genotyped. A significantly different distribution was found in the frequency of the TNC A2128G and C2354T genotypes, but not in T5344A and G6337C genotypes, between the oral cancer and control groups. The G allele TNC A2128G and C allele of C2354T conferred a significant (P<0.05) increased risk of oral cancer. Patients carrying GA or GG of their TNC A2128G, or carrying CT or CC of their TNC C2354T were also of lower 5-year survival, higher metastasis and recurrence rates (P<0.05). Our results provide the first evidence that the G allele of the TNC A2128G and C allele of C2354T may both be associated with the development of oral cancer and may be novel useful biomarkers for oral cancer detection and diagnosis.

doi:1016/j.oos.2009.06.531

P3.07. The association of Alpha B-Crystallin polymorphisms with oralcancer susceptibility in Taiwan

C.L. Chang^a,*, N.W. Chang^a,b, Y.A. Tsou^a, C.F. Chiu^a,1, M.H. Tsai^a,1, D.T. Bau^a,c,1

^aTerry Fox Cancer Research Laboratory, China Medical University Hospital, Taichung, Taiwan

^bDepartment of Biochemistry, School of Medicine, China Medical University, Taichung, Taiwan

^cGraduate Institute of Chinese Medical Science, China Medical University, Taichung, Taiwan, ROC

Alpha B-Crystallin (CRYAB) is an extremely stable protein functions as “molecular chaperone” in preservation intracellular architecture, cell membrane and highly antiapoptotic. Lack or lower CRYAB expression is a prognostic biomarker for head and neck cancer, while its genomic variations and the association with carcinogenesis are not well studied. Therefore, we hypothesized that single nucleotide polymorphisms (SNPs) in CRYAB may be associated with risk of oral cancer. In this hospital-based study, the association of CRYAB A-1215G (rs2228387), C-802G (rs14133) and intron2 (rs2070894) polymorphisms with oral cancer risk in a central Taiwanese population was investigated. In total, 496 patients with oral cancer and 496 age- and gender-matched healthy controls recruited from the China Medical Hospital in central Taiwan were genotyped. A significantly different distribution was found in the frequency of the CRYAB C-802G genotype, but not in A-1215G and intron2 genotypes, between the oral cancer and control groups. The G allele CRYAB C-802G conferred a significant (P=0.000142) increased risk of oral cancer. Patients carrying CG or GG of their CRYAB C-802G were also of lower 5-year survival and higher metastasis rates (P<0.05). Our results provide the first evidence that the G allele of the CRYAB C-802G may be associated with the development of oral cancer and may be a novel useful marker for oral cancer detection and diagnosis.

doi:10.1016/j.oos.2009.06.533

P3.12. Interaction of EXO1 genotypes and smoking habit in oral cancer susceptibility in Taiwan

R.F. Wang^a,*, C.F. Chiu^a, C.S. Liu^a,b, C.C. Lin^b,2, M.H. Tsai^a,2, D.T. Bau^a,c,2

^aTerry Fox Cancer Research Laboratory, China Medical University Hospital, Taichung, Taiwan

^bDepartment of Family Medicine, China Medical University Hospital, Taichung, Taiwan

^cGraduate Institute of Chinese Medical Science, China Medical University, Taichung, Taiwan, ROC

Exonuclease 1 (Exo1) is an important nucleases involved in mismatch repair system that contributes to maintain genomic stability, to modulate DNA recombination, and to mediate cell cycle arrest. Potentially polymorphisms in Exo1 may alter cancer risks by influencing the repair activity of Exo1. Therefore, we hypothesized that single nucleotide polymorphisms (SNPs) in Exo1 were associated with risk of oral cancer. In this hospital-based study, the association of Exo1 A-1419G (rs3754093), C-908G (rs10802996), A238G (rs1776177), C498T (rs1635517), K589E (rs1047840), G670E (rs1776148), C723R (rs1635498), L757P (rs9350) and C3114T (rs851797) polymorphisms with oral cancer risk in a central Taiwanese population was investigated. In total, 680 patients with oral cancer and 680 age- and gender-matched healthy controls recruited from the China Medical Hospital in central Taiwan were genotyped. A significantly different distribution was found in the frequency of the Exo1 K589E genotype, but not the other genotypes, between the oral cancer and control groups. The A allele Exo1 K589E conferred a significant (P=6.18E−8) increased risk of oral cancer. Gene-environment interactions with smoking were significant for Exo1 K589E polymorphism. Our results provide the first evidence that the A allele of the Exo1 K589E may be associated with the development of oral cancer and may be a novel useful marker for primary prevention and anticancer intervention.

doi:10.1016/j.oos.2009.06.538

P3.18. Oral cancer susceptibility is associated with XRCC4 polymorphisms, smoking and betel quid chewing in Central Taiwan

Y.S. Kuo^a,b,*, C.H. Wang^a, C.H. Chang^a, C.F. Chiu^a,3, M.H. Tsai^a,3, D.T. Bau^a,b,c,3

^aTerry Fox Cancer Research Laboratory, China Medical University Hospital, Taichung, Taiwan

^bDepartment of Biological Science and Technology, China Medical University, Taichung, Taiwan

^cGraduate Institute of Chinese Medical Science, China Medical University, Taiwan, ROC

The DNA double strand break repair gene XRCC4, an important caretaker of genome stability, is suggested to play a role in the development of human carcinogenesis. However, no evidence has been provided showing that XRCC4 was associated with oral oncology. In this hospital-based case-control study, the association of XRCC4 G-1394T (rs6869366), intron 3 (rs28360071), intron 7 (rs28360317), and intron 7 (rs1805377) polymorphisms with oral cancer risk in a Taiwanese population was first investigated. In total, 318 patients with oral cancer and 318 age- and gender-matched healthy controls were genotyped. We found a significant different distribution in the frequency of the XRCC4 intron 3 genotype, but not the XRCC4 G-1394T or intron 7 genotypes, between the oral cancer and control groups. Those who had heterozygous del/ins at XRCC4 intron 3 showed a 1.57-fold (95% confidence interval=1.12–2.21) increased risk of oral cancer compared to those with ins/ins. As for XRCC4 G-1394T or intron 7 polymorphisms, there was no difference in the distribution between the oral cancer and control groups. There were significant gene–environment interactions between XRCC4 intron 3 genotype with smoking and with betel quid chewing, but not with alcoholism. In smoker and betel quid chewer groups, the XRCC4 intron 3 del variants exhibited 2.57- and 3.03-fold higher risks than the ins genotype, respectively. Our results firstly suggest that the XRCC4 intron 3 del genotype may be associated with oral oncology and may be a novel useful marker for primary prevention and anticancer intervention.

doi:10.1016/j.oos.2009.06.544

P3.23. Association of oral cancer susceptibility and DNA double strand break gene Ku70 single nucleotide polymorphisms

C.W. Tsai^a,b,*, H.C. Wang^a, C.H. Hua^a, C.F. Chiu^a,4, M.H. Tsai^a,4, D.T. Bau^a,b,4

^aTerry Fox Cancer Research Laboratory, China Medical University Hospital, Taichung, Taiwan

^bGraduate Institute of Chinese Medical Science, China Medical University, Taichung, Taiwan, ROC

The DNA repair gene Ku70, an important caretaker of the overall genome stability, is thought to play a major role in the DNA double strand break repair system. It is known that defects in double strand break repair capacity can lead to irreversible genomic instability. However, the polymorphic variants of Ku70 and their association with oral cancer susceptibility has never been reported on. In this hospital-based case-control study, the association of Ku70 promoter T-991C (rs5751129), promoter G-57C (rs2267437), promoter A-31G (rs132770), and intron3 (rs132774) polymorphisms with oral cancer risk in a Taiwanese population was investigated. In total, 318 patients with oral cancer and 318 age- and gender-matched healthy controls recruited from the China Medical Hospital in Taiwan were genotyped. The results showed that there were significant differences between the oral cancer and control groups in the distribution of their genotypes (P=0.0031) and allelic frequency (P=0.0009) in the Ku70 promoter T-991C polymorphism. Individuals who carried at least one C allele (T/C or C/C) had a 2.15-fold increased risk of developing oral cancer compared to those who carried the T/T wild-type genotype (95% CI: 1.37–3.36). In the other three polymorphisms, there was no difference between both groups in the distribution of either genotype or allelic frequency. In conclusion, the Ku70 promoter T-991C, but not the Ku70 promoter C-57G, promoter A-31G or intron3, is connected to oral cancer susceptibility. This polymorphism may be a novel useful marker for primary prevention and anticancer intervention.

doi:10.1016/j.oos.2009.06.549

P3.49. Effects of gene-environmental interaction of Ku80 with areca chewing on oral cancer susceptibility in Taiwan

H.S. Chen^a,b,*, C.F. Hsu^a, C.F. Chiu^a, Calvin Y.C. Chen^a,b,5, M.H. Tsai^a,5, D.T. Bau^a,b,c,5

^aTerry Fox Cancer Research Laboratory, China Medical University Hospital, Taichung, Taiwan

^bDepartment of Biological Science and Technology, China Medical University, Taichung, Taiwan

^cGraduate Institute of Chinese Medical Science, China Medical University, Taiwan, ROC

The DNA double strand break repair gene Ku80, is thought to play a major role in the caretaking of the overall genome stability. It is very possible that defective in double strand break repair capacity can lead to human carcinogenesis. Thus, the polymorphic variants of Ku80, was firstly investigated about their association with oral cancer susceptibility. In this hospital-based case-control study, the association of Ku80 promoter G-1401T (rs828907), promoter C-319T (rs11685387), and intron19 (rs9288518) polymorphisms with oral cancer risk in a Taiwanese population was investigated. Six hundred patients with oral cancer and 600 age- and gender-matched healthy controls recruited were genotyped and analyzed by PCR-RFLP method. There were significant differences between oral cancer and control groups in the distributions of their genotypes (P=0.0038) and allelic frequencies (P=0.0044) in the Ku80 promoter G-1401T polymorphism. In the other two polymorphisms, there was no difference between both groups in the distribution of either genotype or allelic frequency. There is a synergistic gene-environmental interaction of Ku80 with areca chewing. Compared with G/G genotype in Ku80 promoter G-1401T, the G/T plus T/T significantly enhanced the risk only in the areca chewers (OR=1.603; 95% CI=1.053–2.011), not in the nonareca chewers. In conclusion, the Ku80 promoter G-1401T is corrected with oral cancer susceptibility and this polymorphism may be a useful marker for oral cancer prevention and early detection.

doi:10.1016/j.oos.2009.06.575

• 1 These authors contribted equally to this work.
• 2 These authors contributed equally to this work.
• 3 These authors contributed equally to this work.
• 4 These authors contributed equally to this work.
• 5 These authors contributed equally to this work.