Photodynamic therapy upregulates expression of Mac-1 and generation of leukotriene B4 by human polymorphonuclear leukocytes

Kobayashi, Wataru; Liu, Qiang; Matsumiya, Tomoh; Nakagawa, Hiroshi; Yoshida, Hidemi; Imaizumi, Tadaatsu; Satoh, Kei; Kimura, Hiroto

doi:10.1016/j.oraloncology.2003.10.004

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Volume 40, Issue 5 , Pages 506-510, May 2004

Photodynamic therapy upregulates expression of Mac-1 and generation of leukotriene B₄ by human polymorphonuclear leukocytes

Wataru Kobayashi
- Department of Oral and Maxillofacial Surgery, Hirosaki University School of Medicine, 5-Zaifu-cho, Hirosaki 036-8562, Japan
- Corresponding author. Tel.: +81-172-39-5127; fax: +81-172-39-5128
Qiang Liu
- Department of Oral and Maxillofacial Surgery, The Affiliated Hospital of Stomatology, China Medical University, 117 Nanjing Bei Street, Shenyang 110001, China
Tomoh Matsumiya
- Department of Oral and Maxillofacial Surgery, Hirosaki University School of Medicine, 5-Zaifu-cho, Hirosaki 036-8562, Japan
- Department of Vascular Biology, Institute of Brain Science, Hirosaki University School of Medicine, 5-Zaifu-cho, Hirosaki 036-8562, Japan
Hiroshi Nakagawa
- Department of Oral and Maxillofacial Surgery, Hirosaki University School of Medicine, 5-Zaifu-cho, Hirosaki 036-8562, Japan
Hidemi Yoshida
- Department of Vascular Biology, Institute of Brain Science, Hirosaki University School of Medicine, 5-Zaifu-cho, Hirosaki 036-8562, Japan
Tadaatsu Imaizumi
- Department of Vascular Biology, Institute of Brain Science, Hirosaki University School of Medicine, 5-Zaifu-cho, Hirosaki 036-8562, Japan
Kei Satoh
- Department of Vascular Biology, Institute of Brain Science, Hirosaki University School of Medicine, 5-Zaifu-cho, Hirosaki 036-8562, Japan
Hiroto Kimura
- Department of Oral and Maxillofacial Surgery, Hirosaki University School of Medicine, 5-Zaifu-cho, Hirosaki 036-8562, Japan

Received 8 September 2003; accepted 14 October 2003.

Abstract

This study investigated the expression of Mac-1 (CD11b/CD18) and generation of leukotriene B₄ (LTB₄) by polymorphonuclear leukocytes (PMNs) in response to Mono-L-aspartyl chlorine 6 (NPe6) mediated photodynamic therapy (PDT). PDT was carried out using a laser diode at a light dose of 10 J/cm² and wavelength of 664 nm. Expression of Mac-1 was significantly increased about 3-fold by PDT compared with that of Npe6 exposure. The increase in the expression of Mac-1 was not inhibited by 5-lipoxygenase inhibitor (AA861). On the other hand, generation of LTB₄ by PMNs was increased by incubation with NPe6 alone and further promoted by PDT and that was significantly inhibited by AA861. These results suggest that PDT can induce PMNs to express extensively Mac-1 and generate a certain amount of LTB₄ that may play an important cytocidal role in the treatment of oral cancer.

Keywords: Photodynamic therapy, Mono-L-aspartyl chlorine 6, Polymorphonuclear leukocytes, Mac-1, Leukotriene B₄