Oral Oncology
Volume 40, Issue 5 , Pages 483-489, May 2004

Laminin-1 and SIKVAV a laminin-1-derived peptide, regulate the morphology and protease activity of a human salivary gland adenoid cystic carcinoma cell line

  • V.M Freitas

      Affiliations

    • Department of Histology and Embryology, Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Lineu Prestes 1524, São Paulo SP 05508-900, Brazil
    • ,
  • B Scheremeta

      Affiliations

    • The Pennsylvania State University, University Park, PA, USA
    • ,
  • M.P Hoffman

      Affiliations

    • CDBRB, NIDCR, NIH, Bethesda, MD, USA
    • ,
  • R.G Jaeger

      Affiliations

    • Department of Histology and Embryology, Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Lineu Prestes 1524, São Paulo SP 05508-900, Brazil
    • Corresponding Author InformationCorresponding author. Tel.: 55-11-3091-7742; fax: 55-11-3091-7402

Received 28 August 2003; accepted 1 October 2003.

Abstract 

In a previous paper, we demonstrated that laminin-1 and its derived peptide SIKVAV modulates the morphology of an adenoid cystic carcinoma cell line (CAC2 cells). Light microscopy of CAC2 cells grown in three-dimensional preparations of SIKVAV-enriched laminin-1 showed the presence of pseudocystic spaces. Pseudocysts are hallmarks of adenoid cystic carcinoma in vivo. We hypothesized that these pseudocystic spaces could be due to the protease-inducing/activating role of SIKVAV. Thus, we studied the presence of matrix metalloproteinases (MMPs) in CAC2 cells treated either by laminin-1 or by SIKVAV-enriched laminin-1. Immunohistochemistry and zymography suggested that SIKVAV enhanced the secretion of MMP-2 and MMP-9 in CAC2 cells. We propose that SIKVAV induces pseudocystic formation probably through the secretion of MMPs 2 and 9.

Keywords:  Salivary gland neoplasms, Adenoid cystic carcinoma, Extracellular matrix, Laminin, Matrix metalloproteinases

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PII: S1368-8375(03)00224-0

doi:10.1016/j.oraloncology.2003.10.002

Oral Oncology
Volume 40, Issue 5 , Pages 483-489, May 2004

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