Oral Oncology
Volume 40, Issue 1 , Pages 36-42, January 2004

The effect of laminin and its peptide SIKVAV on a human salivary gland myoepithelioma cell line

  • A.C.T Capuano

      Affiliations

    • Department of Oral Pathology, School of Dentistry, University of São Paulo, São Paulo SP 05508-900, Brazil
    • ,
  • R.G Jaeger

      Affiliations

    • Department of Histology and Embryology, Institute of Biomedical Sciences, University of São Paulo, São Paulo SP 05508-900, Brazil
    • Corresponding Author InformationCorresponding author. Tel.: +55-11-3091-7742; fax: +55-11-3091-7402

Received 6 May 2003; accepted 27 May 2003.

Abstract 

We have demonstrated that the basement membrane regulates the myoepithelioma. We are now studying the effect of laminin, a basement membrane protein, in the morphology of a cell line (M1) derived from human salivary gland plasmacytoid myoepithelioma. These cells were grown inside a three-dimensional preparation of laminin-1. Phenotype differences were assessed by light and transmission electron microscopy. In addition, we analysed the effect of a molecular domain of laminin-1, the peptide SIKVAV, on M1 cells. This peptide was chosen because it is effective in cell proliferation and differentiation. M1 cells grown inside laminin-1 were mostly plasmacytoid-like, while cells treated by SIKVAV showed light and electron microscopic features of typical plasmacytoid cells. This peptide also modulated smooth-muscle actin expression in M1 cells. We demonstrated that laminin-1 and its derived peptide SIKVAV morphoregulates myoepithelioma cells in culture.

Keywords:  Salivary gland neoplasms, Myoepithelioma, Extracellular matrix, Basement membrane, Laminin, Cell culture

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PII: S1368-8375(03)00130-1

doi:10.1016/S1368-8375(03)00130-1

Oral Oncology
Volume 40, Issue 1 , Pages 36-42, January 2004

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