Oral Oncology
Volume 38, Issue 7 , Pages 699-705, October 2002

Molecular characterization of angiogenic properties of human oral squamous cell carcinoma cells

  • Yi-Ju Chen

      Affiliations

    • Institute of Molecular Medicine, National Cheng Kung University Medical College, 1, University Road, Tainan 70101, Taiwan, ROC
    • ,
  • Ying-Tai Jin

      Affiliations

    • Department of Pathology, National Cheng Kung University Medical College, Tainan 70101, Taiwan, ROC
    • ,
  • Dar-Bin Shieh

      Affiliations

    • Department of Medicine, National Cheng Kung University Medical College, Tainan 70101, Taiwan, ROC
    • ,
  • Sen-Tien Tsai

      Affiliations

    • Department of Otolaryngology, National Cheng Kung University Medical College, Tainan 70101, Taiwan, ROC
    • ,
  • Li-Wha Wu

      Affiliations

    • Institute of Molecular Medicine, National Cheng Kung University Medical College, 1, University Road, Tainan 70101, Taiwan, ROC
    • Corresponding Author InformationCorresponding author

Received 22 October 2001; accepted 18 December 2001.

Abstract 

Little is known about the specificity of angiogenic properties of oral cancer cells and the possible mechanisms. Stimulatory effects on proliferation and migration of human umbilical vein endothelial cells (HUVEC) characterized the angiogenic properties of oral cancer cells but not normal oral keratinocytes (NOK). ELISA found the presence of vascular endothelial growth factors (VEGF) both in the tested oral cancer cells and NOK. Attenuation of the proangiogenic effects by neutralizing VEGF antibodies suggests VEGF play a key role in the acquisition of the angiogenic phenotype in oral cancer cells. Western blotting of p53 and murine double mutant 2 (Mdm2) together with p53 DNA sequencing analysis indicate that p53 function loss by mutation or overexpression of Mdm2 occurred in all tested oral cancer cells regardless of their etiology. In summary, the angiogenic property of oral cancer cells is mediated by many factors in addition to VEGF and the functional status of p53.

Keywords:  Oral cancer, Squamous cell carcinoma, Angiogenesis, VEGF, p53, Mdm2, Keratinocytes

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PII: S1368-8375(02)00004-0

Oral Oncology
Volume 38, Issue 7 , Pages 699-705, October 2002

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