Oral Oncology
Volume 38, Issue 3 , Pages 258-265 , April 2002

Prevention of the areca nut extract-induced unscheduled DNA synthesis of gingival keratinocytes by vitamin C and thiol compounds

  • M.C. Chang

      Affiliations

    • Team of Biomedical Science, Chang-Gung Institute of Nursing, Taoyuan, Taiwan
    • ,
  • Y.S. Ho

      Affiliations

    • Institute of Medical Technology, Taipei Medical College, Taipei, Taiwan
    • ,
  • J.J. Lee

      Affiliations

    • Laboratory of Dental Pharmacology and Toxicology, Graduate Institute of Dental Science, National Taiwan University, and Department of Dentistry, National Taiwan University Hospital, No 1, Chang-Te Street, Taipei, Taiwan
    • ,
  • S.H. Kok

      Affiliations

    • Laboratory of Dental Pharmacology and Toxicology, Graduate Institute of Dental Science, National Taiwan University, and Department of Dentistry, National Taiwan University Hospital, No 1, Chang-Te Street, Taipei, Taiwan
    • ,
  • L.J. Hahn

      Affiliations

    • Laboratory of Dental Pharmacology and Toxicology, Graduate Institute of Dental Science, National Taiwan University, and Department of Dentistry, National Taiwan University Hospital, No 1, Chang-Te Street, Taipei, Taiwan
    • ,
  • J.H. Jeng

      Affiliations

    • Laboratory of Dental Pharmacology and Toxicology, Graduate Institute of Dental Science, National Taiwan University, and Department of Dentistry, National Taiwan University Hospital, No 1, Chang-Te Street, Taipei, Taiwan
    • Corresponding Author InformationCorresponding author. Fax: +886-2-23821212

Received 5 April 2001 ,Accepted 2 May 2001.

References 

  1. IARC. Betel-quid and areca nut chewing. Monographs on the evaluation of the carcinogenic risk of chemicals to humans, vol. 37. p. 141–202. Lyon, France, International Agency for Research on Cancer, 1985.
  2. Thomas SJ, MacLennan R. Slaked lime and betel nut cancer in Papua New Guinea. Lancet. 1992;340:577–578
  3. Warnakulasuriya S. The role of betel-quid in oral carcinogenesis. In R. Bedi and P. Jones (Eds), Usage and health issues. Center for transcultural oral health, London, UK, 1995.
  4. Reichart PA, Phillipsen HP. Betel chewer's mucosa — a review. J Oral Pathol Med. 1998;27:239–242
  5. Sharan RN. Association of betel nut with carcinogenesis. Cancer J. 1996;9:13–19
  6. Sharan RN, Wary KK. Study of unscheduled DNA synthesis following exposure of human cells to arecoline and extracts of betel nut in vitro. Mutat Res. 1992;278:271–276
  7. Liu TY, Chcn CL, Chi CW. Oxidative damage to DNA induced by areca nut extract. Mutat Res. 1996;367:25–31
  8. Sundqvist K, Liu Y, Nair J, Bartsch H, Arvidson K, Grafstrom RC. Cytotoxic and genotoxic effects of areca nut related compounds in cultured human buccal epithelial cells. Cancer Res. 1989;49:5294–5298
  9. Sundqvist K, Grafstrom RC. Effects of areca nut on growth, differentiation and formation of DNA damage in cultured human buccal epithelial cells. Int J Cancer. 1992;52:305–310
  10. Jeng JH, Kuo ML, Hahn LJ, Kuo MYP. Genotoxic and non-genotoxic effects of betel quid ingredients on oral mucosal fibroblasts in vitro. J Dent Res. 1994;73:1043–1049
  11. Jeng JH, Hahn LJ, Lin PR, Chan CP, Hsieh CC, Chang MC. Effects of areca nut, Inflorescence piper betle extracts and arecoline on the cytotoxicity, total and unscheduled DNA synthesis of cultured human gingival keratinocytes. J Oral Pathol Med. 1999;28:64–71
  12. Jeng JH, Ho YS, Chan CP, Wang YJ, Lei D, Hsu CC, et al. Areca nut extract upregulates prostaglandins synthesis, cyclooxygenase-2 mRNA and protein production of human oral keratinocytes. Carcinogenesis. 2000;21:1365–1370
  13. Nair UJ, Floyd RA, Nair J, Bussachini V, Friesen M, Bartsch H. Formation of reactive oxygen species and 8-hydroxydeoxyguanosine in DNA in vitro with betel quid ingredients. Chem Biol Interact. 1987;63:157–169
  14. Stich HF, Anders F. The involvement of reactive oxygen species in oral cancers of betel quid/tobacco chewers. Mutat Res. 1989;214:47–61
  15. Stich HF, Stich W, Rosin MP, Vallejera MO. Use of micronucleus test to monitor the effect of vitamin A, beta-carotene and canthaxanthin on the buccal mucosa of betel nut/tobacco chewers. Int J Cancer. 1984;34:745–750
  16. Enwonwu CO, Meeks VI. Bionutrition and oral cancer in humans. Crit Rev Oral Biol Med. 1995;6:5–17
  17. Mosmann T. Rapid colorimetric assay for cellular growth and survival. Application to proliferation and cytotoxicity assay. J Immunol Methods. 1983;65:55–63
  18. Zhu B-Z, Har-El R, Kitrossky N, Chevion M. New modes of action of desferrioxamine: Scavenging of semiquinone radical and stimulation of hydrolysis of tetrachlorohydroquinone. Free Radical Biol Med. 1998;24:360–369
  19. Witte I, Zhu B-Z, Lueken A, Magnani D, Helge A, Chevion M. Protection by desferrioxamine and other hydroxamic acids against tetrachlorohydroquinone-induced cyto- and genotoxicity in human fibroblasts. Free Radical Biol Med. 2000;28:693–700
  20. Hoe S, Rowley DA, Halliwell B. Reactions of ferrioxamine and desferrioxamine with the hydroxyl radical. Chem-Biol Interact. 1982;41:75–81
  21. Halliwell B. Use of desferrioxamine as a “probe” for the iron-dependent formation of hydroxyl radicals. Evidence for a direct reaction between desferal and the superoxide radical. Biochem. Pharmacol. 1985;34:229–233
  22. Cooper CE, Green ESR, Rice-Evans CA, et al.  A hydrogen-donating monohydroxamate scavengers ferryl myoglobin radicals. Free Radical Biol Med. 1994;20:219–222
  23. Patrineli A, Clifford MN, Ioannides C. Contribution of phenols, quinones and reactive oxygen species to the mutagenicity of white grape juice in the ames test. Food Chem Toxicol. 1996;34:869–872
  24. Bolton JL, Trush MA, Penning TM, et al.  Role of quinones in toxicology. Chem Res Toxicol. 2000;13:135–160
  25. Hu JJ, Dubin N, Kurland D, Ma BL, Roush GC. The effect of hydrogen peroxide on DNA repair activity. Mutat Res. 1995;336:193–201
  26. Pero RW, Anderson MW, Doyle GA, Anna CH, Romagna F, Markowitz M, et al. Oxidative stress induces DNA damage and inhibits the repair of DNA lesions induced by N-acetoxy-2-acetylaminofluorene in human peripheral mononuclear leukocytes. Cancer Res. 1990;50:4619–4625
  27. Setlow RB. Repair deficient human disorders and cancer. Nature. 1978;221:713–717
  28. De Vries N, De Flora S. N-acetyl-l-cysteine. J Cell Biochem (Suppl). 1993;17F:270–277
  29. Toyokuni S. Iron-induced carcinogenesis: the role of redox regulation. Free Radical Biol Med. 1996;20:553–566
  30. Paul RR, Chatterjee J, Das AK, Dutta SK, Roy D. Zinc and iron as bioindicators of precancerous nature of oral submucous fibrosis. Biol Trace Element Res. 1996;54:213–230
  31. Wang CK, Su HY, Lii CK. Chemical composition and toxicity of Taiwanese betel quid extract. Food Chem Toxicol. 1999;37:135–144
  32. Keberle H. The biochemistry of desferrioxamine and its relation to iron metabolism. Ann NY Acad Sci. 1964;119:758–768
  33. Mans DRA, Lafleur MVM, Westmizje IR, Horn IR, Bets D, Schuurhuis GJ, et al. Reactions of glutathione with the catechol, the ortho-quinone and the semiquinone free radical of etoposide. Biochem. Pharmacol. 1992;43:1761–1768
  34. Lunte SM, Kissinger PT. Detection and identification of sulfhydryl conjugates of p-benzoquinones in microsomal incubations of benzene and phenol. Chem Biol Interact. 1983;47:195–212
  35. Kapadia GK, Chung EB, Ghosh B, Shukla YN, Basak SP, Morton JF, et al. Carcinogenicity of some folk-medicinal herbs in rats. J Natl Cancer Institute. 1978;60:683–686
  36. Ranadive KJ, Ranadive SN, Shivapurkar NM, Gothoskar SV. Betel quid chewing and oral cancer: experimental studies on hamsters. Int J Cancer. 1979;24:835–843
  37. Dunham LJ, Herrold KM. Failure to produce tumors in the hamster cheek pouch by exposure to ingredients of betel quid: histopathologic changes in the pouch and other organs by exposure to known carcinogens. J Natl Cancer Institute. 1962;29:1047–1067
  38. Lee CS, Hartley JA, Berardini MD, Butler J, Soegel D, Ross D, et al. Alteration in DNA crosslinking and sequence selectivity of a series of aziridinyl-benzoquinones after enzymatic reduction by DT-diaphorase. Biochemistry. 1991;31:3019–3025
  39. Nair UJ, Nair J, Friesen MD, Bartsch H, Ohshima H. Ortho- and meta-tyrosine formation from phenylalanine in human saliva as a marker of hydroxyl radical generation during betel quid chewing. Carcinogenesis. 1995;16:1195–1198
  40. Jeng JH, Lan WH, Hahn LJ, Hsieh CC, Kuo MYP. Inhibition of the migration, attachment, spreading, growth and collagen synthesis of human gingival fibroblasts by arecoline, a major areca alkaloid, in vitro. J Oral Pathol Med. 1996;25:371–375
  41. Wong DYK, Hsiao YL, Poon CK, Kwan PC, Chao SY, Chou ST, et al. Glutathione concentration in oral cancer tissues. Cancer Lett. 1994;81:111–116
  42. Flagg EW, Coates RJ, Jones DP, et al.  Dietary glutathione intake and the risk of oral and pharyngeal cancer. Am J Epidemiol. 1994;139:453–465

PII: S1368-8375(01)00053-7

Oral Oncology
Volume 38, Issue 3 , Pages 258-265 , April 2002

Article Tools

* Image Usage & Resolution