Regulation of cell motility via high and low affinity autocrine motility factor (AMF) receptor in human oral squamous carcinoma cells

Niinaka, Y.; Haga, A.; Negishi, A.; Yoshimasu, H.; Raz, A.; Amagasa, T.

« Previous Next »Oral Oncology
Volume 38, Issue 1 , Pages 49-55, January 2002

Regulation of cell motility via high and low affinity autocrine motility factor (AMF) receptor in human oral squamous carcinoma cells

Y. Niinaka
- Tumour Progression and Metastasis Research Program, Karmanos Cancer Institute, 110 East Warren Avenue, Detroit, MI 48201, USA
- Maxillofacial Surgery, Graduate School, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan
- Department of Dental and Oral Surgery, Yachimata General Hospital, 137-1, Yachimata-Ho, Yachimata, Chiba 289-1115, Japan
- Corresponding author. Tel.: +81-3-5803-5503; fax: +81-3-5803-0198
A. Haga
- Tumour Progression and Metastasis Research Program, Karmanos Cancer Institute, 110 East Warren Avenue, Detroit, MI 48201, USA
- Department of Public Health, Gifu Pharmaceutical University, 5-6-1 Mitahora-Higashi, Gifu 502-8585, Japan
A. Negishi
- Present address. Department of Oral and Maxillofacial Surgery, School of Medicine, Gunma University, 3-39-22 Showa, Maebashi-shi, Gunma 371-8511, Japan.
- Maxillofacial Surgery, Graduate School, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan
H. Yoshimasu
- Maxillofacial Surgery, Graduate School, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan
A. Raz
- Tumour Progression and Metastasis Research Program, Karmanos Cancer Institute, 110 East Warren Avenue, Detroit, MI 48201, USA
T. Amagasa
- Maxillofacial Surgery, Graduate School, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan

Received 4 December 2000; accepted 13 December 2000.

Abstract

A tumour-secreted cytokine autocrine motility factor (AMF) induces in vivo invasion and metastasis, and in vitro tumour cell motility by a signal transduction through interaction with its cell surface receptor gp78. In this report, we investigated the characterization of a high-metastatic human oral squamous cell carcinoma (SCC) cell line LMF4 and low-metastatic HSC-3 in comparison with non-metastatic HSC-2 and HSC-4. Morphological and motility analyses revealed LMF4 cells to have the highest motile activity among those cells. However, LMF4 cells shared the similar features with HSC-3: high level secretion of AMF, enhancement of gp78 expression, co-expression of vimentin and cytokeratin, although LMF4 cells showed twice as high motile reactivity as HSC-3. The only difference was that LMF4 had twice as high amount of low-affinity receptor(s) as HSC-3, shown by Scatchard analysis.

Keywords: Tumour, Cancer, Carcinoma, Motility, Locomotion, Migration, Autocrine, Receptor, Factor, Expression