Oral Oncology
Volume 37, Issue 6 , Pages 521-526 , September 2001

In situ staining with DNA-binding fluorescent dye, Hoechst 33258, to detect microorganisms in the epithelial cells of oral leukoplakia

Received 4 January 2001 ,Accepted 17 January 2001.

References 

  1. Miller CS, White DK. Human papillomavirus expression in oral mucosa, premalignant conditions, and squamous cell carcinoma. A retrospective review of the literature. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1996;82:57–68
  2. Mao E-J, Scwartz SM, Daling JR, Oda D, Tickman L, Beckmann AM. Human papilloma viruses and p53 mutations in normal, pre-malignant and malignant oral epithelia. Int J Cancer. 1996;69:152–158
  3. D'Costa J, Saranath D, Dedhia P, Sanghvi V, Mehta AR. Detection of HPV-16 genome in human oral cancers and potentially malignant lesions from India. Oral Oncology. 1998;34:413–420
  4. Sand L, Jalouli J, Larsson PA, Hirsch JM. Human papilloma viruses in oral lesions. Anticancer Res. 2000;20:1183–1188
  5. Hashimoto K, Richard J, DiBella RJ, Tarnowski WM, Shklar G. Electron microscopic studies of oral benign leukoplakia. Oral Surg. 1968;25:901–913
  6. Banoczy J, Juhasz J, Albrecht M. Ultrastructure of different clinical forms of oral leukoplakias. J Oral Pathol. 1980;9:41–53
  7. Kannan S, Balaram P, Pillai MR, Chandran GJ, Nair MK, Kartha CC, et al. Ultrastructural variations and assessment of malignant transformation risk in oral leukoplakia. Path Res Pract. 1993;189:1169–1180
  8. Hayward AF. Membrane coating granules. Int Rev Cytol. 1979;59:97–127
  9. Wertz PW, Squier CA. Cellular and molecular basis of barrier function in oral epithelium. Crit Rev Ther Drug Carrier Syst. 1991;8:237–269
  10. Stokke T, Steen HB. Multiple binding modes for Hoechst 33258 to DNA. J Histochem Cytochem. 1985;33:333–338
  11. Chen TR. In situ detection of mycoplasma contamination in cell cultures by fluorescent Hoechst 33258 stain. Exp Cell Res. 1977;104:255–262
  12. Hessling JJ, Miller SE, Levy NL. A direct comparison of procedures for the detection of mycoplasma in tissue culture. J Immunol Methods. 1980;38:315–324
  13. Battaglia M, Pozzi D, Grimaldi S, Parasassi T. Hoechst 33258 staining for detecting mycoplasma contamination in cell cultures (a method for reducing fluorescence photobleaching). Biotech Histochem. 1994;69:152–156
  14. Masover GK, Becker FA. Detection of mycoplasmas in cell cultures by fluorescence methods. Methods Molec Biol. 1998;104:217–226
  15. Grossgebauer K, Rolly H. Fluorescent, DNA-binding dyes for rapid detection of Chlamydia trachomatis. Microsc Acta. 1982;86:1–11
  16. Laube U, Kiderlen AF. Detection of parasites with DNA-binding bisbenzimide H33258 in Pneumocystis carinii- and Leishmania-containing materials. Parasitol Res. 1998;84:559–564
  17. Muller W, Gautier F. Interactions of heteromatic compounds with nucleic acids. A-T specific non-intercalating DNA-ligands. Eur J Biochem. 1975;54:385–394
  18. Harshman KD, Dervan PB. Molecular recognition of B-DNA by Hoechst 33258. Nucleic Acids Res. 1985;13:4825–4835
  19. Portugal J, Waring MJ. Assignment of DNA binding sites for 4′,6-diamidine-2-phenylindole and bisbenzimide (Hoechst 33258). A comparative footprinting study. Biochem Biophys Acta. 1988;949:158–168
  20. Haq I, Ladbury JE, Chowdhry BZ, Jenkins TC, Chaires JB. Specific binding of hoechst 33258 to the d(CGCAAATTTGCG)2 duplex (calorimetric and spectroscopic studies). J Molec Biol. 1997;271:244–257
  21. Belton CM, Eversole LR. Oral hairy leukoplakia (ultrastructural features). J Oral Pathol. 1986;15:493–499
  22. Kanas RJ, Abrams AM, Jensen JL, Wuerker RB, Handlers JP. Oral hairy leukoplakia (ultrastructural observations). Oral Surg Oral Med Oral Pathol. 1988;65:333–338
  23. Greenspan JS, Rabanus j-P, Petersen V, Greenspan D. Fine structure of EBV-infected keratinocytes in oral hairy leukoplakia. J Oral Pathol Med. 1989;18:565–572
  24. Toki T, Oikawa N, Tase T, Sato S, Wada Y, Yajima A, et al. Immunohistochemical and electron microscopic demonstration of human papillomavirus in dysplasia of the uterine cervix. Tohoku J Exp Med. 1986;149:163–167
  25. Shroyer KR, Greer RO. Detection of human papillomavirus DNA by in situ DNA hybridization and polymerase chain reaction in premalignant and malignant oral lesions. Oral Surg Oral Med Oral Pathol. 1991;71:708–713
  26. Zeuss MS, Miller CS, White DK. In situ DNA hybridization analysis of human papillomavirus DNA in oral mucosal lesions. Oral Surg Oral Med Oral Pathol. 1991;71:714–720
  27. Kumagai K, Iwabuchi T, Hinuma Y, Yuri K, Ishida N. Incidence, species, and significance of mycoplasma species in the mouth. J Infect Dis. 1971;123:16–21
  28. Watanabe T, Matsuura M, Seto K. Enumeration, isolation, and species identification of mycoplasmas in saliva sampled from the normal and pathological human oral cavity and antibody response to an oral mycoplasma (Mycoplasma salivarium). J Clin Microbiol. 1986;23:1034–1038
  29. Willett HP. Mycoplasma. In:  Joklik WK,  Willett HP,  Amos DB,  Wilfert CM editor. Zinsser microbiology, 20th ed. London: Prentice-Hall International; 1992;p. 730–737
  30. Clyde WA, Chanock RM, Tully JG. Mycoplasmas. In:  Davis BD,  Dulbecco R,  Eisen HN,  Ginsberg HS editor. Microbiology, 4th ed. Philadelphia: Lippincott; 1990;p. 707–711
  31. Rottem S, Naot Y. Subversion and exploitation of host cells by mycoplasmas. Trends Microbiol. 1998;6:436–440
  32. Rottem S, Barile MF. Beware of mycoplasmas. Trends Biotechnol. 1993;11:143–151

PII: S1368-8375(01)00016-1

Oral Oncology
Volume 37, Issue 6 , Pages 521-526 , September 2001

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