Oral Oncology
Volume 37, Issue 6 , Pages 537-544 , September 2001

5-Fluorouracil induces autophagic degeneration in rat oral keratinocytes

  • I von Bültzingslöwen

      Affiliations

    • Department of Endodontology and Oral Diagnosis, Faculty of Odontology, University of Göteborg, Box 450, S-405 30 Göteborg, Sweden
    • Corresponding Author InformationCorresponding author. Tel.: +46-31-7733251; fax: +46-31-825416
    • ,
  • M Jontell

      Affiliations

    • Department of Endodontology and Oral Diagnosis, Faculty of Odontology, University of Göteborg, Box 450, S-405 30 Göteborg, Sweden
    • ,
  • P Hurst

      Affiliations

    • Department of Anatomy and Structural Biology, University of Otago School of Medicine, Great King Street, Dunedin, New Zealand
    • ,
  • U Nannmark

      Affiliations

    • Institute of Anatomy and Cell Biology, Faculty of Medicine, University of Göteborg, Box 420, S-405 30 Göteborg, Sweden
    • ,
  • T Kardos

      Affiliations

    • Department of Oral Sciences and Orthodontics, University of Otago School of Dentistry, Great King Street, Dunedin, New Zealand

Received 8 October 2000 ,Accepted 4 November 2000.

References 

  1. Bültzingslöwen Iv, Jontell M. Macrophages, dendritic cells and T lymphocytes in rat buccal mucosa and dental pulp following 5-fluorouracil treatment. Eur J Oral Sci. 1999;107:194–201
  2. Guggenheimer J, Verbin RS, Appel BN, Schmutz J. Clinicopathologic effects of cancer chemotherapeutic agents on human buccal mucosa. Oral Surg Oral Med Oral Pathol. 1977;44:58–63
  3. Lockhart PB, Sonis ST. Alterations in the oral mucosa caused by chemotherapeutic agents. A histologic study. J Dermatol Surg Oncol. 1981;7:1019–1025
  4. Sonis ST. Mucositis as a biological process (a new hypothesis for the development of chemotherapy-induced stomatotoxicity). Oral Oncol. 1998;34:39–43
  5. Clarkson JE, Worthington HV, Eden OB. Prevention of oral mucositis or oral candidiasis for patients with cancer receiving chemotherapy (excluding head and neck cancer) (Cochrane Review). In: The Cochrane library. Oxford: Update Software, 2000;1.
  6. Epstein JB, Schubert MM. Oral mucositis in myelosuppressive cancer therapy. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1999;88:273–276
  7. Plevova P. Prevention and treatment of chemotherapy- and radiotherapy-induced oral mucositis (a review). Oral Oncol. 1999;35:453–470
  8. Ijiri K, Potten CS. Response of intestinal cells of differing topographical and hierarchical status to ten cytotoxic drugs and five sources of radiation. Br J Cancer. 1983;47:175–185
  9. Pritchard DM, Potten CS, Hickman JA. The relationships between p53-dependent apoptosis, inhibition of proliferation, and 5-fluorouracil-induced histopathology in murine intestinal epithelia. Cancer Res. 1998;58:5453–5465
  10. Pritchard DM, Potten CS, Korsmeyer SJ, Roberts S, Hickman JA. Damage-induced apoptosis in intestinal epithelia from bcl-2-null and bax-null mice (investigations of the mechanistic determinants of epithelial apoptosis in vivo). Oncogene. 1999;18:7287–7293
  11. Tong D, Poot M, Hu D, Oda D. 5-Fluorouracil-induced apoptosis in cultured oral cancer cells. Oral Oncol. 2000;36:236–241
  12. Gundersen HJ. Stereology of arbitrary particles. A review of unbiased number and size estimators and the presentation of some new ones, in memory of William R. Thompson. J Microsc. 1986;143:3–45
  13. Clarke PG. Developmental cell death (morphological diversity and multiple mechanisms). Anat Embryol (Berl). 1990;181:195–213
  14. Gavrieli Y, Sherman Y, Ben-Sasson SA. Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J Cell Biol. 1992;119:493–501
  15. Charriaut-Marlangue C, Ben-Ari Y. A cautionary note on the use of the TUNEL stain to determine apoptosis. Neuroreport. 1995;7:61–64
  16. Grasl-Kraupp B, Ruttkay-Nedecky B, Koudelka H, Bukowska K, Bursch W, Schulte-Hermann R. In situ detection of fragmented DNA (TUNEL assay) fails to discriminate among apoptosis, necrosis, and autolytic cell death (a cautionary note). Hepatology. 1995;21:1465–1468
  17. Dive C, Gregory CD, Phipps DJ, Evans DL, Milner AE, Wyllie AH. Analysis and discrimination of necrosis and apoptosis (programmed cell death) by multiparameter flow cytometry. Biochim Biophys Acta. 1992;1133:275–285
  18. Huschtscha LI, Jeitner TM, Andersson CE, Bartier WA, Tattersall MH. Identification of apoptotic and necrotic human leukemic cells by flow cytometry. Exp Cell Res. 1994;212:161–165
  19. Swat W, Ignatowicz L, Kisielow P. Detection of apoptosis of immature CD4+8+ thymocytes by flow cytometry. J Immunol Methods. 1991;137:79–87
  20. Cao S, Lu K, Toth K, Slocum HK, Shirasaka T, Rustum YM. Persistent induction of apoptosis and suppression of mitosis as the basis for curative therapy with S-1, an oral 5-fluorouracil prodrug in a colorectal tumor model. Clin Cancer Res. 1999;5:267–274
  21. Kitanaka C, Kuchino Y. Caspase-independent programmed cell death with necrotic morphology. Cell Death Differ. 1999;6:508–515
  22. Zakeri Z, Bursch W, Tenniswood M, Lockshin A. Cell death (programmed, apoptosis, necrosis, or other?). Cell Death Diff. 1995;2:87–96
  23. Zakeri ZF, Quaglino D, Latham T, Lockshin RA. Delayed internucleosomal DNA fragmentation in programmed cell death. Faseb J. 1993;7:470–478
  24. Zakeri Z, Quaglino D, Ahuja HS. Apoptotic cell death in the mouse limb and its suppression in the hammertoe mutant. Dev Biol. 1994;165:294–297
  25. Yang WCT, Srasser FF, Pomerat CM. Mechanism of drug-induced vacuolization in tissue culture. Exp Cell Res. 1965;38:495–506
  26. Belkin M, Hardy WG, Orr HC, Lachman AB. Induction in vitro by autonomic drugs of cytoplasmic vacuoles in ascites tumor cells. J Natl Cancer Inst. 1962;28:187–201
  27. Henics T, Wheatley DN. Cytoplasmic vacuolation, adaptation and cell death (a view on new perspectives and features). Biol Cell. 1999;91:485–498
  28. Alberts B, Bray D, Lewis J, Raff M, Roberts K, Watson JD. Differentiated cells and the maintenance of tissues. Molecular biology of the cell, New York: Garland Publishing, 1994. p. 1139–95.

PII: S1368-8375(01)00009-4

Oral Oncology
Volume 37, Issue 6 , Pages 537-544 , September 2001

Article Tools

* Image Usage & Resolution