Oral Oncology
Volume 37, Issue 4 , Pages 341-344 , June 2001

Selective deletion of p14(ARF) exon 1β of the INK4a locus in oral squamous cell carcinomas of Indians

  • M Viswanathan

      Affiliations

    • Cancer Biology Division, School of Biological Sciences, Madurai Kamaraj University, Madurai - 625 021, India
  • ,
  • N Tsuchida

      Affiliations

    • Department of Molecular and Cellular Oncology and Microbiology, Tokyo Medical and Dental University, Tokyo 113, Japan
  • ,
  • G Shanmugam

      Affiliations

    • Cancer Biology Division, School of Biological Sciences, Madurai Kamaraj University, Madurai - 625 021, India
    • Corresponding Author InformationCorresponding author. Tel.: +91-452-859126; fax: +91-452-858228

Received 4 September 2000 ,Accepted 27 September 2000.

References 

  1. Kamb A, Gruis NA, Weaver-Feldhaus J, et al.  A cell cycle regulator potentially involved in genesis of many tumor types. Science. 1994;264:436–440
  2. Serrano M, Hannon GJ, Beach D. A new regulatory motif in cell-cycle control causing specific inhibition of cyclin D/CDK4. Nature. 1993;366:704–707
  3. Quelle DE, Zindy F, Ashmun RA, Sherr CJ. Alternative reading frames of the INK4a tumor suppressor gene encode two unrelated proteins capable of inducing cell cycle arrest. Cell. 1995;83:993–1000
  4. Serrano M, Lee HW, Chin L, Cordon-Cardo C, Beach D, DePinho RA. Role of the INK4a locus in tumor suppression and cell immortality. Cell. 1996;85:27–37
  5. Stott F, Bates S, James MC, et al.  The alternative product from the human CDKN2A locus, p14(ARF), participates in a regulatory feedback loop with p53 and MDM2. EMBO J. 1998;17:5001–5014
  6. Fountain JW, Karayiorgou M, Ernstoff MS, et al.  Homozygous deletions within human chromosome band 9p21 in melanoma. Proc. Nat. Acad. Sci. 1992;89:10557–10561
  7. Kamb A, Shattuck-Eidens D, Eeles R, et al.  Analysis of the p16 gene (CDKN2) as a candidate for the chromosome 9p melanoma susceptibility locus. Nature Genet. 1994;8:22–26
  8. Ranade K, Hussussian CJ, Sikorski RS, et al.  Mutations associated with familial melanoma impair p16(INK4) function. Nature Genet. 1995;10:114–116
  9. Caldas C, Hahn SA, da Costa LT, et al.  Frequent somatic mutations and homozygous deletions of the p16(MTS1) gene in pancreatic adenocarcinoma. Nature Genet. 1994;8:27–32
  10. Liu Q, Yan YX, McClure M, Nagakawa H, Fujimora F, Rustgi AK. MTS-1 (CDKN2) tumor suppressor gene deletions are a frequent event in esophagus squamous cancer and pancreatic adenocarcinoma cell lines. Oncogene. 1995;10:619–622
  11. Ogawa S, Hirano N, Sato N, et al.  Homozygous loss of the cyclin-dependent kinase 4-inhibitor (p16) gene in human leukemias. Blood. 1994;84:2431–2435
  12. Hatta Y, Hirama T, Miller CW, Yamada Y, Tomonaga M, Koeffler HP. Homozygous deletion of the p15(MTS2) and p16(MTS1) genes in adult T-cell leukemia. Blood. 1995;85:2699–2704
  13. Igaki H, Sasaki H, Kishi T, et al.  Highly frequent homozygous deletion of the p16 gene in esophageal cancer cell lines. Biochem Biophys. Res. Commun. 1994;203:1090–1095
  14. Pomerantz J, Schreiber-Agus N, Liegeois NJ, et al.  The Ink4 tumor suppressor gene product, p19(ARF), interacts with MDM2 and neutralizes MDM2’s inhibition of p53. Cell. 1998;92:713–723
  15. Tao W, Levine AJ. p19 (ARF) stabilizes p53 by blocking nucleo-cytoplasmic shuttling of MDM2. Proc. Natl. Acad. Sci. 1999;96:6937–6941
  16. Zhang Y, Xiong Y, Yarbrough WG, et al.  ARF promotes MDM2 degradation and stabilizes p53 (ARF-INK4a locus deletion impairs both the Rb and p53 tumor suppression pathways). Cell. 1998;92:725–734
  17. Zhang Y, Xiong Y. Mutations in human ARF exon 2 disrupt its nucleolar localization and impair its ability to block nuclear export of MDM2 and p53. Molec. Cell. 1999;3:579–591
  18. Sherr CJ, Weber JD. The ARF/p53 pathway. Curr. Opin. in Genet. and Dev. 2000;10:94–99
  19. Orlow I, Rabbani F, Chin L, et al.  Involvement of the INK4a gene (p16 and p19arf) in murine tumorigenesis. Int. J. Oncol. 1999;15:17–24
  20. Tsutsumi M, Tsai YC, Gonzalgo ML, Nichols PW, Jones PA, et al.  Early acquisition of homozygous deletions of p16/p19 during squamous cell carcinogenesis and genetic mosaicism in bladder cancer. Oncogene. 1998;17:3021–3027
  21. Stadler WM, Olopade OI. The 9p21 region in bladder cancer cell lines (large homozygous deletion inactivate the CDKN2, CDKN2B and MTAP genes). Urol. Res. 1996;24:239–244
  22. Herranz M, Urioste M, Santos J, et al.  Analysis of the INK4a/ARF locus in non-Hodgkin’s lymphomas using two new internal microsatellite markers. Leukemia. 1999;13(5):808–810
  23. Maniatis T, Fritsch EF, Sambrook J. Molecular cloning: a laboratory manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory; 1989;
  24. Gazzeri S, Della-Valle V, Chaussade L, Brambilla C, Larsen CJ, Brambilla E. The Human p19(ARF) protein encoded by the β transcript of the p16 gene is frequently lost in small cell lung cancer. Cancer Res. 1998;58:3926–3931
  25. Kannan K, Munirajan AK, Krishnamurthy JK, et al.  The p16 Ink4a/p19 ARF gene mutations are infrequent and are mutually exclusive to p53 mutations in Indian oral squamous cell carcinomas. Int. J. Oncology. 2000;16:585–590
  26. Kannan K, Munirajan AK, Krishnamurthy JK, et al.  Low incidence of p53 mutations in betel quid and tobacco chewing -associated oral squamous carcinoma from India. Int. J. Oncology. 1999;15:1133–1136
  27. Bishop JA, Harland M, Bennet DC, et al.  Mutation testing in melanoma families (INK4A, CDK4 and INK4D). Br. J. Cancer. 1999;80:295–300
  28. Quelle DE, Cheng M, Ashmun RA, Sherr CJ. Cancer associated mutations at the INK4 locus cancel cell cycle arrest by p16(INK4a) but not by the alternative reading frame protein p19 (ARF). Proc. Nat. Acad. Sci. 1997;94:669–673

PII: S1368-8375(00)00112-3

Oral Oncology
Volume 37, Issue 4 , Pages 341-344 , June 2001